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一个糖化血红蛋白在CX5上的参数,老提示定标失败,不知道错在哪里,请高手帮忙指点,不过俺没有金币可赠,只有空感谢!
反应特性:试剂1+样本,3-5分钟,读空白吸光度1,然后加试剂2,反应5分钟读吸光度2,进行计算。定标类型,4参数对数曲线,定标点:5点(空白+4个标准液)
主要参数如下:
Reaction type: endpoint 2
reaction direction: positive
math model: 1
No. of calibrators:5
calibrator 1: 0.00
2: 4.30
3: 8.10
4: 14.20
5: 22.40
6: 0.00
primary wavelength: 340
secondary wavelength: 700
reagents (primary inject): [A] vol:200 ul [NONE] vol: 0 ul
reagents (secondary inject): [B] vol: 40 ul Add time: 592 sec
sample volume: 8 ul
reagent blank: reaction:
----------------- --------------------------
start read: 500 sec start read: 600 sec
end read: 540 sec end read: 640
substrate depletion:
initial rate: 99.999 delta abs: 1.500
multipoint span
1-2: 0.000
2-3: 0.000
3-4: 0.000
4-5: 0.000
recovery/sensitivity
std dev (conc): 0.00
cv(%): 0.00
std dev (mA): 0.00
threshold: 9999.99
定标结果在 5个Reaction的吸光度后面都有一个“*”
提示:
-----------------------------------------
FAILED CALIBRATION (*)
LEVEL CONDITION MAGNITUDE
1 SPAN 0.08637
2 SPAN 0.10187
3 SPAN 0.10433
4 SPAN 0.05293
5 SPAN 034550
请高手帮忙看看啊,谢谢!谢谢!
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