Sysmex RAM-1全自动网织红细胞计数的评价 [转帖]-->yeec转移

　　Reticulocyte counting using the Sysmex RAM-1 was evaluated. The results of 113 samples analysed on the Sysmex RAM-1 were compared with those from both flow cytometry (FCM) and a manual technique.Reticulocyte counting with the Sysmex RAM-1 showed excellent precision,with an overall coefficient of variance (CV) less than 5%,and almost no carry-over.Reticulocyte counts were stable after blood storage for 48 h at 4℃ and room temperature (RT). Linearity was demonstrated very well for Sysmex RAM-1 and FCM (rRAM=0.9998,rFCM=0.9974,P<0.001) when reticulocyte counts were 5×109/1-480×109/l.Comparisons of methods showed a good relation for reticulocyte counts between Sysmex RAM-1 and manual counting (r=0.9760),FCM and manual counting (r=0.9623),and Sysmex RAM-1 and FCM (r=0.9527).Analysis of receiver operating characteristic curves showed that the true-positive rate (TPR) was 0.95 for Sysmex RAM-1,and 0.83 for FCM; the area under curve was 0.999 and 0.972 for Sysmex RAM-1,0.990 and 0.900 for FCM.These findings confirm that Sysmex RAM-1 can make counting reticulocytes easier,more accurate and more reproducible. 彭黎明 杨惠 江虹等 详见：Clin.Lab.Haem.2001,23,97-102