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伯乐Bio-Rad550酶标仪通讯协议

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赵光 发表于 2007-12-14 20:31 | 显示全部楼层 |阅读模式

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RS-232 Interface Specifications

The following specification describes the syntax and language required for computer control

of the reader. In addition, the Model 550 reader will perform passive unidirectional data

transmission. The instrument will automatically transmit the data in ASCII code after each

reading if it is properly connected to a host computer. See the “RTPLATE” command for an

example of ASCII output.

Note: This automatic output will be of Measurement O.D. minus Reference O.D. when

in Dual Wavelength mode (i.e. only one set of 96 numbers).

1. SIGNALS

TXD: transmit data

RXD: receive data

DTR: data terminal ready

DSR: data set ready

CTS: clear to send

RTS: ready to send

SIGNAL GROUND (SHIELD GROUND to hood / chassis ground)

2. TRANSMISSION

Baud rate 9600

Data width 8

Stop width 1

Parity None

3. DATA

All data must be transmitted in ASCII.

The READER command interpreter must be “case blind” (no distinction between

upper or lower case ASCII characters).

All READER commands can be distinguished from each other by the first two

characters of the command.

4. SIGNAL ASSIGNMENT

Pin # READER Signal type Host Signal Pair

1 SHIELD GROUND SHIELD GROUND

2 TRANSMIT DATA RECEIVE DATA

3 RECEIVE DATA TRANSMIT DATA

4 REQUEST TO SEND CLEAR TO SEND

5 CLEAR TO SEND REQUEST TO SEND

6 DATA SET READY DATA TERMINAL READY

7 SIGNAL GROUND SIGNAL GROUND

20 DATA TERMINAL READY DATA SET READY

Shield ground 1

Transmit data 2

Receive data 3

Data set ready 6

Signal GND 7

Data terminal ready 20

Clear to send 5

Request to send 4

Host

Computer

Model 550

Reader

Command Language for the Model 550 Microplate Reader

SYNTAX:<device name><space><command>[ <space><command args.>] <cr>

device name: “EIA. READER” -Model 550 Microplate READER

1. <command>: “ID”-request device ID. 550 READER id; “550”; no arguments

<response>: “ERE” <space><error code><space><id><cr>

<ID>: “0550”: Model 550 Microplate READER ID code]

2. <command>: “AQ” - Acquire remote control of the device and lock front panel

key pad; no arguments

When acquired, the reader will be in the Remote Control mode. The

LCD will display Remote Control mode, and the printer will automatically

be deactivated. The reader will stay in the remote control mode

until the release (RL) command is received or the STOP button on the

front panel is pressed.

<response>: “ERE” <space><error code><cr>

3. <command>: “RL”- Release remote control of the device and release front panel key

pad; no arguments

<response>: “ERE” <space><error code><cr>

4. <command>: “RS”- Reset device to power up configuration local mode; no arguments

<response>: “ERE”<space><error code><cr>

5. <command>: “MR”- Transmit maintenance report; no arguments

<response>: “ERE” <space><error code><cr><mrecords><cr>

< >: maintenance records;

“On/off:” <#on/off><cr> :Number of times the reader was turned on.

“Hours:” <#hours><cr> :Number of hours the reader was on.

“Plates:” <#plates><cr> :Number of plates read.

Note: <#on/off>,<#hours> and <#plates> are 4 ASCII encoded decimal values. This

information is kept in battery backup RAM.

6. <command>: “RM”- Reset maintenance report values; no arguments

<response>: “ERE” <space><error code><cr>

7. <command>: “RWELL”- Read a plate well; arguments <c1>, <rw>, <s/d>

<cmd args>: cl - Column number ASCII encoded decimal (1-12)

rw - Row number ASCII encoded decimal (1-8)

wp1 - Measurement filter position ASCII encoded decimal (1-4)

wp2 - Reference filter position ASCII encoded decimal (1-4)

Optional; only present for Dual wavelength reading

<response>: “ERE” <space><error code><space><abs. val1)

<space><optional abs. val2 for reference wavelength><cr>

<abs. val1>: Absorbance values at measurement wavelength (ASCII encoded

floating point format) ex.” 1.234”

<abs val2>: Absorbance values at reference wavelength (ASCII encoded floating

point format) ex.”1.234”

33

8. <command>: “RPLATE” - read plate; arguments <mix>, <wp1>,<wp2>

<cmd args>: mix - Mixing time before reading (in seconds) ; ASCII encoded

decimal (0-9)

wp1 - Measurement filter position; ASCII encoded decimal (1-4)

wp2 - Reference filter position; ASCII encoded decimal (1-4)

Optional; only present for Dual wavelength reading

<response>: “ERE” <space><error code><space><abs. data><cr>

9. <command>: “RTPLATE” - retransmit the last plate read; no arguments

<response>: “ERE” <space><error code><space><abs. data><cr>

Absorbance Data

<abs. data>: <header><filter info>< Mes.data><cr> or <header><filter info>

<Mes. data><cr><Ref.data><cr> Ref. data present for dual wavelength readings.

<header>: “BIO-RAD MODEL 550 READER”<cr>

<filter info>: “Mes. filter:” <wp1><cr>

“Ref. filter:” <wp2><cr> - Present for Dual wavelength reading

<Mes. data>: “ . begin” <cr><8 rows of 12 wells of abs. values><checksum><cr>

“.end” <cr>

Measurement wavelength absorbance values are in ASCII encoded

floating point format. Each absorbance value is separated by a space.

Row1=wells (A1-A12), Row2=wells (B1-B12), .... Row8=wells (H1-

H12).

<Ref. data>: “ . begin” <cr><8 rows of 12 wells of abs. values><checksum><cr>

“.end” <cr>

Reference wavelength absorbance values are in ASCII encoded

floating point format. Each absorbance value is seperated by a space.

Row1=wells (A1-A12), ROW2=wells (B1-B12, ....Row8=wells (H1-

H12)

<checksum>: The checksum is a two byte sum in ASCII encoded decimal (0-255)

of all the ASCII characters transmitted in the measurement or

reference data block. The checksum does not include the character

string “.begin”<cr> in the checksum calculations.

The following is an example of both <Mes. data> and <Ref. data>:

“. begin”<cr>

“ 0.101 0.102 0.103 0.104 0.105 0.106 0.107 0.108 0.109 0.110 0.111 0.112”<cr>

“ 0.201 0.202 0.203 0.204 0.205 0.206 0.207 0.208 0.209 0.210 0.211 0.212”<cr>

“ 0.301 0.302 0.303 0.304 0.305 0.306 0.307 0.308 0.309 0.310 0.311 0.312”<cr>

“ 0.401 0.402 0.403 0.404 0.405 0.406 0.407 0.408 0.409 0.410 0.411 0.412”<cr>

“ 0.501 0.502 0.503 0.504 0.505 0.506 0.507 0.508 0.509 0.510 0.511 0.512”<cr>

“ 0.601 0.602 0.603 0.604 0.605 0.606 0.607 0.608 0.609 0.610 0.611 0.612”<cr>

“ 0.701 0.702 0.703 0.704 0.705 0.706 0.707 0.708 0.709 0.710 0.711 0.712”<cr>

“ 0.801 0.802 0.803 0.804 0.805 0.806 0.807 0.808 0.809 0.810 0.811 0.812”<cr>

<checksum><cr>

“ . end”<cr>

Note: Absorbance values greater then 3.000 shall be transmitted as an asterisk.

34

Error Codes

“0000” - No error

“8071” - Invalid Command

“8072” - Parameter Out of Range

“8073” - Device Not In Remote Mode

“8074” - Device Busy

“8075” - Not assigined

“8076” - Not assigined

“8077” - Light Bulb Burned Out

“8078” - Hardware Error

“8079” - Memory Error

“8080” - Not assigined

“8081” - Not assigined

Note: Until the Model 550 Reader receives the “AQ” command, the reader will be unable

to accept any other command and will only respond with the error code of “8073”- Device Not

In Remote Mode.

Once the reader accepts the “AQ” command, the mode will be turned into the “Remote

Mode” and then the front key pad will be locked until pressing “START/STOP” key or accepting

“RL” command.

 


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