Sysmex SE-9500测定外周血干细胞的研究 [转帖]-->yeec转移

　　The Sysmex SE-9500 automated haematology analyser provides an estimate of immature cells,referred to as'haematopoietic progenitor cells'(HPC),The aim of this study was to evaluate the reliability and usefulness of the SE-9500 HPC parameter as compared with the CD34+cell count and to determine whether the HPC count was of value in predicting the optimal harvesting time for peripheral blood stem cells (PBSC).Studies were performed on 112 samples form 21 patients with haematological malignancies and 13 healthy donors undergoing progenitor cell mobilisation.Coefficients of variation for the HPC count were 30%,23.8%,12.4% and 8.3% respectively for samples with low (4×106)，medium (13×106/l),high (250×106/l) and very high (2413×6/I) counts.There was good linearity for HPC measurement in both peripheral blood (PB) and purified CD34+cell suspensions (r>0.995), and no detectable carryover was obsered.There was and acceptable correlation between HPC and CD34+cell counts for PB samples (r=0.669) and for CD34+cell suspensions (r=0.859). Analysis of purified CD34+cells using the SE-9500 HPC mode revealed that they appear both in the blast cell area and the immature granulocyte area of the analyser cell display. Quantitation of CD34+cells and HPC during PBSC mobilisation showed good agreement between these parameters with regard to the optimal time for PBSC harvesting.These findings suggest that HPC counting with the Sysmex SE-9500 may be clinically useful for optimising the timing of PBSC collection. 彭黎明 杨佳荟 杨杰等 详见：Clin.Lab.Haem.2001,23,231-236