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[转帖]酶标仪参数对照

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lzjq68 发表于 2005-10-9 10:04 | 显示全部楼层 |阅读模式

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  Thermo Labsystems Thermo Labsystems Thermo Labsystems Thermo Labsystems Thermo Labsystems BioTek BioTek Molecular Devices Molecular Devices Tecan Tecan BioRad BioRad BioRad Asys Hitech Asys Hitech Asys Hitech
Instrument Multiskan Ascent MRX Revelation iEMS Reader MF Multiskan EX & RC Opsys MR ELx800 ELx808 Vmax Emax Sunrise Standard model Sunrise Gradient filter model 550 Benchmark Ultramark DigiScan Expert 384 Jupiter
User interface On-board     On-board On-board On-board On-board On-board On-board On-board Win based sw On-board Win based sw On-board On-board        
  PC PC PC PC PC PC PC PC PC PC or touchscreen PC or touchscreen PC PC PC PC PC PC
Plate type 96, 384 96 96 96 96 24,48, 96, 384 as option 96 96 96 96   96 96 1-1536-well 96 384 96, 48, 24 on request 12, 24, 48, 96 optional 484
Wavelength separation Interference filters Interference filters Interference filters Interference filters Interference filters Interference filters Interference filters Interference filters Interference filters Filter slide with 4 fixed filters Gradient filter slide Filters Filters Filters Interference filters Interference filters Interference filters
Wavelength range 340-850 340-850 340-850 400-750 405-690 400-750 nm 380-900 400-750 nm 400-750 nm 340-750 400-700 400-750 340-750 250-750 400-800 400-800 400-800
            340 - (option) 340- option               340-800 (option) 340-800 340-800
Photometric range 0-6 -0.1-4 0-6 0-3.5 0-3.5 0-3   0-4 Abs 0-4 Abs 0-3 Abs (340-399 nm)   0-3.5 0-4 Abs 0-4.5 0-3.2 0-3 0-3.7
                    0-4 Abs (400-750 nm)              
Accuracy ±1% (0-2 Abs) ±2.5% ±1% (0-2 Abs) ±2% ±2.5% (0-3Abs) ±1% (0-2 Abs) ±1% (0-2.5 Abs)     ±1% (0-2 Abs, 492 nm) ±1.5% (0-2 Abs, 492 nm) ±1.5% at 1.0 Abs ±1.0% (0-4 Abs, 490 nm) < ±0.5% (0-4 Abs) ±1.0% at 1.5 Abs ±1.0% at 1.5 Abs ±1.0%
  ±2% (2-3 Abs)   ±2% (2-3 Abs)       ±2% (2.5-3.5 Abs)     ±1.5% (2-3 Abs, 492 nm)              
Precision CV <0.2% 0.3–3 Abs , CV<1.0% (3-4) stepping CV 0.2% Abs (0–2.0 Abs) CV <0.2% (0–3 Abs) , CV<1.0% (3-4 Abs) CV <0.5% +/-0.01 Abs (0–0.5 Abs) ±0.5% (0-2 Abs) ±0.5% (0-2.5 Abs)     ±0.5% (0-2 Abs, 492 nm) ±1% (0-2.5 Abs, 492 nm) 1% (0-2 Abs) <1% (400-750 nm, 0-3 Abs) < ±0.5% (0-4 Abs) ±0.5% at 1.5 Abs ±0.5% at 1.5 Abs ±0.5%
  CV <0.3%, 0.3-2 Abs, CV<1% (2-3Abs), continuous CV 0.6% (2.0–3.0 Abs) CV <1.0% (0.5–2.0 Abs)   ±1.5% (2.5-3.5 Abs)     ±1% (2-3 Abs, 492 nm)     <2% (340-399 nm, 0-2 Abs)      
    CV 1.0% (3.0–3.5 Abs)     CV <1.5% (2.0–3.0 Abs)               <3.5% (400-700 nm, 3-4 Abs)        
Linearity 0-4 Abs, +/-2%, stepping, 96-well 0–2.0 Abs, +/-1.0% 0-4 Abs, +/-1%, stepping 0-2.5 Abs 0–2.5 Abs, +/-1.0% ±1% (0-2 Abs) ± 1% (0-2.5 Abs)     ± 2% (0-2 Abs, 340-399 nm) ± 2% (0-2.5 Abs, 492 nm) 2% (0-3 Abs) 2% error (0-2 Abs, 340-400 nm) ± 1% (0-4 Abs, 405 nm)      
  0-3 Abs, +/-2%, continuous, 96-well 2-3.5 Abs, +/-1.5% 0-3 Abs, +/-1%, continuous 2.5-3 Abs, +/-1.5% ±3% (2-3 Abs) ± 2% (2.5-3.5 Abs)     ± 1% (0-2 Abs, 400-750 nm)     2% error (0-3 Abs, 401-450 nm)      
  0-3 Abs, +/-2%, stepping, 384-well               2% error (0-4 Abs, 451-500 nm)      
  0-2 Abs, +/-2%, continuous, 384-well                                
Reading speed 9 s/96 well , 20 s/384 well plate, cont. <4 s 3 s, continuous mode 5 s <10 s 30 s 12 s 6 s 5 s 6 s 6 s Not specified 7 s 6 s / 96-well 5 s 5 s 25 s
  14 s/96 well, 42 s/384 well, stepping 6 s, stepping mode               12 s / 384-well    
                      33 s / 1536-well    
Incubation yes Optional Yes No No No Yes   No Yes Yes No Yes Yes Yes, option    
Range Amb +4 - 50 Amb to 63 Amb +3 - 40   Amb +4-50           Amb+5 - 42 Amb + 4 to 45  
Uniformity +/-0.8 across the plate stability: <+/- 0.5 +/-0.2 across the plate   ± 0.5 oC                
Accuracy   +/- 1.0 +/-0.5                       ± 0.4    
Shaking Yes, linear Yes Yes, orbital Yes Yes No Yes Yes No Yes, linear Yes, linear Yes Yes Yes, 3 speeds   Yes, integral Yes, integral
                                   
Max. Filters 8 6 8 8 6 5           4 6 10 6 6 6
Filters included     8     4 4               4 4-5 4
Robotic integration Yes No No No No Yes Yes     Yes Yes     Yes   Yes Yes
Light source halogen                         Xenon Flash   Halogen  
Photodetector           1 photodiode 8 photodiodes, 1 ref                    
                                   
Read method Endpoint, Endpoint, Endpoint, Endpoint, Endpoint Endpoint Endpoint, Endpoint, Endpoint Endpoint Endpoint Endpoint Endpoint Endpoint      
  Kinetics Kinetics Kinetics Kinetics     Slow kinetics Kinetics   Kinetics Kinetics   Kinetic Kinetic      
Dispenser     Yes, 20-390 µl, 8 channels                            
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