这是翻译的问题,其实换个思路就很好理解。
第一个,向下反应中,下限是最大的反应度就好理解了。反应度用吸光度数值来表示,向下反应自然是开始比结束高,结束时的吸光度自然是最大的反应度。但绝对不能在设置吸光度的时候,下限比上限高。还是跟上升反应一样,下限低于上限的吸光度。
第二个,也是翻译问题,其中“的回应”是多余的,而且校正剂也过度翻译,原文是CAL。其实就是如果CAL2的数据小于CAL1的数据,多点跨距必须为负号。这个也很好理解,六点定标,加入1-2,2-3,3-4,4-5,5-6都是递增(或递减)关系的话,多点跨距就应该是+(或者-)。但到了6-1肯定就会出现逆转,所以这一段的多点跨距就是-(或者+)。
原文如下:
Reaction (Absorbance or Rate)
Reaction specifies the minimum and maximum absorbance or rate (method dependent) measuredduring the reaction read window.
• For upgoing rate or endpoint reactions, if the reaction absorbance is less than the Low Limitthen results are suppressed and flagged as RX ABS LO. Likewise, if the High Limit is exceeded,the result is suppressed and flagged RX ABS HI.
• For downgoing rate methods, the Low Limit indicates the maximum rate observed during thereaction read window.
• For downgoing endpoint methods, the Low Limit indicates the maximum absorbance observedduring the reaction read window.
Mean deviation is a measure of noise in the read window.
Table 8.28 Allowable Entries for Reaction
Low Limit -1.500 to 2.200
High Limit -1.500 to 2.200
Mean Deviation 0.000 to 2.200
Multipoint Spans
Multipoint span limits specify the allowable difference in absorbance or rate between respectivemultipoint calibrator levels:
• 1 and 2
• 2 and 3
• 3 and 4
• 4 and 5
• 5 and 6
• 6 and 1
Values are only shown for the number of calibrators specified under "# of Calibrators". A change incalibrator number resets the spans to zero (0.000).
The sign (positive or negative) of multipoint spans are dependent upon the direction of changebetween two successive calibrators. For example, if the response for Cal 2 data < Cal 1 data, themultipoint span must be negative.
Table 8.30 Allowable Entries for Multipoint Span Limits
-1.500 to 2.200
IMPORTANT The system may accept incorrect calibration data if:
• multipoint span values are not entered during UDR setup,
• span values of 0 are entered, and/or
• the samples are not loaded correctly during the run.
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